A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.

ABSTRACT:

Background

The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high efficiency. Despite the efforts have made on Artemisia annua transient expression, achieving high-throughput gene functional characterization basing on a fast and easy-manipulated transient transformation system in A. annua remains challenging.

Results

The first pair of true leaves of A. annua is an ideal candidate for Agrobacterium injection. EHA105 was the optimal strain that can be used for the development of the transient expression system. The supplementation of Triton X-100 at a concentration of 0.005% greatly improved the transient expression frequenc

SUBMITTER: Li Y 

PROVIDER: S-EPMC8520255 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature