Prevalence of Helicobacter pylori Antimicrobial Resistance Among Chilean Patients.

ABSTRACT:

Background

Treatments for Helicobacter pylori (H. pylori) eradication include the use of antibiotics and a proton-pump inhibitor. Antibiotic resistance is a major concern for two drugs: levofloxacin and clarithromycin. The aim was to determine the prevalence of levofloxacin resistance (LevoR) and clarithromycin resistance (ClaR) in an urban population in Santiago, Chile.

Methods

Gastric mucosa biopsies were obtained for DNA isolation from 143 H. pylori-positive individuals aged 18-80 years. Direct sequencing of the quinolone-resistance determining region (QRDR) of the gyrA gene was used to determine LevoR. ClaR was determined using restriction-fragment length polymorphism or 5'exonuclease assay.

Results

The prevalences of LevoR and ClaR were 29 and 27%, respectively.

SUBMITTER: Gonzalez-Hormazabal P 

PROVIDER: S-EPMC8520490 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature