Integrative proteome analysis implicates aberrant RNA splicing in impaired developmental potential of aged mouse oocytes.

ABSTRACT: Aging has many effects on the female reproductive system, among which decreased oocyte quality and impaired embryo developmental potential are the most important factors affecting female fertility. However, the mechanisms underlying oocyte aging are not yet fully understood. Here, we selected normal reproductively aging female mice and constructed a protein expression profile of metaphase II (MII) oocytes from three age groups. A total of 187 differentially expressed (DE) proteins were identified, and bioinformatics analyses showed that these DE proteins were highly enriched in RNA splicing. Next, RNA-seq was performed on 2-cell embryos from these three age groups, and splicing analysis showed that a large number of splicing events and genes were discovered at this stage. Differentially sp

SUBMITTER: Li M 

PROVIDER: S-EPMC8520726 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature