Creation of a synthesis-friendly inflammation-inducible promoter suitable for cell therapy.

ABSTRACT: The development of 'smart' cell-based therapeutics requires cells that first recognize conditions consistent with disease (e.g. inflammation) and then subsequently release therapeutic proteins, thereby reducing potential toxicity from otherwise continuous expression. Promoters containing NF-κB response elements are often used as reporters of inflammation; however, endogenous promoters have crosstalk with other pathways, and current synthetic promoters have many exact sequence repeats of NF-κB response elements which make them both difficult to synthesize and inherently genetically unstable. Herein, a synthesis-friendly inflammation-inducible promoter (named SFNp) was created by the packing of 14 NF-κB response elements, which have no repeats >9 bp, followed by a minimal cytomegalovirus pro

SUBMITTER: Jadav A 

PROVIDER: S-EPMC8521041 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature