Single-Cell RNA Sequencing Revealed CD14⁺ Monocytes Increased in Patients With Takayasu's Arteritis Requiring Surgical Management.

ABSTRACT: Objectives: Takayasu Arteritis (TA) is a highly specific vascular inflammation and poses threat to patients' health. Although some patients have accepted medical treatment, their culprit lesions require surgical management (TARSM). This study aimed at dissecting the transcriptomes of peripheral blood mononuclear cells (PBMCs) in these patients and to explore potential clinical markers for TA development and progression. Methods: Peripheral blood were collected from four TA patients requiring surgical management and four age-sex matched healthy donors. Single cell RNA sequencing (scRNA-seq) was adopted to explore the transcriptomic diversity and function of their PBMCs. ELISA, qPCR, and FACS were conducted to validate the results of the analysis. Results: A total of 299

SUBMITTER: Qing G 

PROVIDER: S-EPMC8521054 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature