Real-time three-dimensional tracking of single vesicles reveals abnormal motion and pools of synaptic vesicles in neurons of Huntington's disease mice.

ABSTRACT: Although defective synaptic transmission was suggested to play a role in neurodegenerative diseases, the dynamics and vesicle pools of synaptic vesicles during neurodegeneration remain elusive. Here, we performed real-time three-dimensional tracking of single synaptic vesicles in cortical neurons from a mouse model of Huntington's disease (HD). Vesicles in HD neurons had a larger net displacement and radius of gyration compared with wild-type neurons. Vesicles with high release probability (P_r) were interspersed with low-P_r vesicles in HD neurons, whereas high-P_r vesicles were closer to fusion sites than low-P_r in wild-type neurons. Non-releasing vesicles in HD neurons had an abnormally high prevalence of irregular oscillatory motion. These abnor

SUBMITTER: Chen S 

PROVIDER: S-EPMC8521108 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature