Altered Intestinal Permeability and Drug Repositioning in a Post-operative Ileus Guinea Pig Model.

ABSTRACT:

Background/aims

The aim of this study is to identify the alteration in intestinal permeability with regard to the development of post-operative ileus (POI). Moreover, we investigated drug repositioning in the treatment of POI.

Methods

An experimental POI model was developed using guinea pigs. To measure intestinal permeability, harvested intestinal membranes of the ileum and proximal colon was used in an Ussing chamber. To identify the mechanisms associated with altered permeability, we measured leukocyte count and expression of calprotectin, claudin-1, claudin-2, and mast cell tryptase. We compared control, POI, and drug groups (mosapride [0.3 mg/kg and 1 mg/kg, orally], glutamine [500 mg/kg, orally], or ketotifen [1 mg/kg, orally] with regard to these parameters.

Result

SUBMITTER: Kim YM 

PROVIDER: S-EPMC8521477 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature