Responsiveness to perturbations is a hallmark of transcription factors that maintain cell identity in vitro.

ABSTRACT: Identifying the particular transcription factors that maintain cell type in vitro is important for manipulating cell type. Identifying such transcription factors by their cell-type-specific expression or their involvement in developmental regulation has had limited success. We hypothesized that because cell type is often resilient to perturbations, the transcriptional response to perturbations would reveal identity-maintaining transcription factors. We developed perturbation panel profiling (P³) as a framework for perturbing cells across many conditions and measuring gene expression responsiveness transcriptome-wide. In human iPSC-derived cardiac myocytes, P³ showed that transcription factors important for cardiac myocyte differentiation and maintenance were among the

SUBMITTER: Mellis IA 

PROVIDER: S-EPMC8522198 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature