Effects of sequence motifs in the yeast 3' untranslated region determined from massively parallel assays of random sequences.

ABSTRACT:

Background

The 3' untranslated region (UTR) plays critical roles in determining the level of gene expression through effects on activities such as mRNA stability and translation. Functional elements within this region have largely been identified through analyses of native genes, which contain multiple co-evolved sequence features.

Results

To explore the effects of 3' UTR sequence elements outside of native sequence contexts, we analyze hundreds of thousands of random 50-mers inserted into the 3' UTR of a reporter gene in the yeast Saccharomyces cerevisiae. We determine relative protein expression levels from the fitness of transformants in a growth selection. We find that the consensus 3' UTR efficiency element significantly boosts expression, independent of sequence contex

SUBMITTER: Savinov A 

PROVIDER: S-EPMC8522215 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature