The Major Peanut Allergen Ara h 2 Produced in <i>Nicotiana benthamiana</i> Contains Hydroxyprolines and Is a Viable Alternative to the <i>E. Coli</i> Product in Allergy Diagnosis.

ABSTRACT: Peanut allergy is a potentially life-threatening disease that is mediated by allergen-specific immunoglobulin E (IgE) antibodies. The major peanut allergen Ara h 2, a 2S albumin seed storage protein, is one of the most dangerous and potent plant allergens. Ara h 2 is posttranslationally modified to harbor four disulfide bridges and three hydroxyprolines. These hydroxyproline residues are required for optimal IgE-binding to the DPYSP^OHS motifs representing an immunodominant IgE epitope. So far, recombinant Ara h 2 has been produced in Escherichia coli, Lactococcus lactis, Trichoplusia ni insect cell, and Chlamydomonas reinhardtii chloroplast expression systems, which were all incapable of proline hydroxylation. However, molecular diagnosis of peanut allergy is perfo

SUBMITTER: Uzulmez O 

PROVIDER: S-EPMC8522509 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature