Unique anticodon loop conformation with the flipped-out wobble nucleotide in the crystal structure of unbound tRNA^Val.

ABSTRACT: During protein synthesis on ribosome, tRNA recognizes its cognate codon of mRNA through base-pairing with the anticodon. The 5'-end nucleotide of the anticodon is capable of wobble base-pairing, offering a molecular basis for codon degeneracy. The wobble nucleotide is often targeted for post-transcriptional modification, which affects the specificity and fidelity of the decoding process. Flipping-out of a wobble nucleotide in the anticodon loop has been proposed to be necessary for modifying enzymes to access the target nucleotide, which has been captured in selective structures of protein-bound complexes. Meanwhile, all other structures of free or ribosome-bound tRNA display anticodon bases arranged in stacked conformation. We report the X-ray crystal structure of unbound tRNA^Val1

SUBMITTER: Jeong H 

PROVIDER: S-EPMC8522699 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature