Exploring STR sequencing for forensic DNA intelligence databasing using the Austrian National DNA Database as an example.

ABSTRACT: Here, we present the results from a population study that evaluated the performance of massively parallel sequencing (MPS) of short tandem repeats (STRs) with a particular focus on DNA intelligence databasing purposes. To meet this objective, 247 randomly selected reference samples, earlier being processed with conventional capillary electrophoretic (CE) STR sizing from the Austrian National DNA Database, were reanalyzed with the PowerSeq 46Y kit (Promega). This sample set provides MPS-based population data valid for the Austrian population to increase the body of sequence-based STR variation. The study addressed forensically relevant parameters, such as concordance and backward compatibility to extant amplicon-based genotypes, sequence-based stutter ratios, and relative marker performance

SUBMITTER: Holzl-Muller P 

PROVIDER: S-EPMC8523457 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature