Using Live-Cell Imaging and Synthetic Biology to Probe Directed Migration in Dictyostelium.

ABSTRACT: For decades, the social amoeba Dictyostelium discoideum has been an invaluable tool for dissecting the biology of eukaryotic cells. Its short growth cycle and genetic tractability make it ideal for a variety of biochemical, cell biological, and biophysical assays. Dictyostelium have been widely used as a model of eukaryotic cell motility because the signaling and mechanical networks which they use to steer and produce forward motion are highly conserved. Because these migration networks consist of hundreds of interconnected proteins, perturbing individual molecules can have subtle effects or alter cell morphology and signaling in major unpredictable ways. Therefore, to fully understand this network, we must be able to quantitatively assess the consequences of abrupt modifications. T

SUBMITTER: Kuhn J 

PROVIDER: S-EPMC8523838 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature