Somatostatin Neurons in the Mouse Pontine Nucleus Activate GABA_A Receptor Mediated Synaptic Currents in Locus Coeruleus Neurons.

ABSTRACT: The pontine nuclei comprising the locus coeruleus (LC) and Barrington's nucleus (BRN) amongst others form the neural circuitry(s) that coordinates arousal and voiding behaviors. However, little is known about the synaptic connectivity of neurons within or across these nuclei. These include corticotropin-releasing factor (CRF⁺) expressing neurons in the BRN that control bladder contraction and somatostatin expressing (SST⁺) neurons whose role in this region has not been discerned. To determine the synaptic connectivity of these neurons, we employed optogenetic stimulation with recordings from BRN and LC neurons in brain stem slices of channelrhodopsin-2 expressing SST or CRF neurons. Optogenetic stimulation of CRF⁺ BRN neurons of Crf ^Cre

SUBMITTER: Garcia DuBar S 

PROVIDER: S-EPMC8524133 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature