Visualizing transfer of microbial biomolecules by outer membrane vesicles in microbe-host-communication in vivo.

ABSTRACT: The intestinal microbiota influences mammalian host physiology in health and disease locally in the gut but also in organs devoid of direct contact with bacteria such as the liver and brain. Extracellular vesicles (EVs) or outer membrane vesicles (OMVs) released by microbes are increasingly recognized for their potential role as biological shuttle systems for inter-kingdom communication. However, physiologically relevant evidence for the transfer of functional biomolecules from the intestinal microbiota to individual host cells by OMVs in vivo is scarce. By introducing Escherichia coli engineered to express Cre-recombinase (E. coli^Cre ) into mice with a Rosa26.tdTomato-reporter background, we leveraged the Cre-LoxP system to report the transfer of bacterial OMVs to recipient cel

SUBMITTER: Bittel M 

PROVIDER: S-EPMC8524437 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature