Dataset Information

Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain.

ABSTRACT: Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain and spine disorders. The development of ciliated cells, their impact on CSF flow, and their function in brain and axial morphogenesis are not fully understood. We have characterized motile ciliated cells within the zebrafish brain ventricles. We show that the ventricles undergo restructuring through development, involving a transition from mono- to multiciliated cells (MCCs) driven by gmnc. MCCs co-exist with monociliated cells and generate directional flow patterns. These ciliated cells have different developmental origins and are genetically heterogenous with respect to expression of the Foxj1 family of ciliary master regulators. Finally, we show that cilia loss from the tela choroida and choroid plexus or global perturbation of multiciliation does not affect overall brain or spine morphogenesis but results in enlarged ventricles. Our findings establish that motile ciliated cells are generated by complementary and sequential transcriptional programs to support ventricular development.

SUBMITTER: D'Gama PP

PROVIDER: S-EPMC8524669 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain.

D'Gama Percival P PP Qiu Tao T Cosacak Mehmet Ilyas MI Rayamajhi Dheeraj D Konac Ahsen A Hansen Jan Niklas JN Ringers Christa C Acuña-Hinrichsen Francisca F Hui Subhra P SP Olstad Emilie W EW Chong Yan Ling YL Lim Charlton Kang An CKA Gupta Astha A Ng Chee Peng CP Nilges Benedikt S BS Kashikar Nachiket D ND Wachten Dagmar D Liebl David D Kikuchi Kazu K Kizil Caghan C Yaksi Emre E Roy Sudipto S Jurisch-Yaksi Nathalie N

Cell reports 20211001 1

Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain and spine disorders. The development of ciliated cells, their impact on CSF flow, and their function in brain and axial morphogenesis are not fully understood. We have characterized motile ciliated cells within the zebrafish brain ventricles. We show that the ventricles undergo restructuring through development, involving a transition from mono- to multiciliated cells (MCCs) driven by gmnc. MCCs co-exist with monocili ...[more]

PMID: 34610312

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Project description:Species and populations can be categorized by their sexual systems, depending on the spatial distribution of female and male reproductive structures within and among plants. Although a high diversity of sexual systems exists in Silene, their relative frequency at the genus and infrageneric level is unknown. Here, we carried out an extensive literature search for direct or indirect descriptions of sexual systems in Silene species. We found descriptions of sexual systems for 98 Silene species, where 63 and 35 correspond to the phylogenetically supported subgenera Silene and Behenantha, respectively. Hermaphroditism was the commonest sexual system (58.2 %), followed by dioecy (14.3 %), gynodioecy (13.3 %) and gynodioecy-gynomonoecy (i.e. hermaphroditic, female and gynomonoecious plants coexisting in the same population; 12.2 %). The presence of these sexual systems in both subgenera suggests their multiple origins. In 17 species, the description of sexual systems varied, and in most cases these differences corresponded to variations within or among populations. Interestingly, the poorly studied gynodioecy-gynomonoecy sexual system showed similar frequency to dioecy and gynodioecy in both subgenera. In addition, the incidence of gynodioecy-gynomonoecy was analysed in the species of section Psammophilae (Silene littorea, S. psammitis, S. adscendens and S. cambessedesii), in a survey of 26 populations across the distribution area of the species. The four species showed gynomonoecy-gynodioecy in most populations. Hermaphrodites were the most frequent morph, with a low number of females and gynomonoecious plants in all populations. The frequency of sexual morphs varied significantly among the studied populations but not among species. Female plants generally produced smaller numbers of flowers than hermaphroditic or gynomonoecious plants, and the percentages of female flowers per population were low. All these findings suggest that the gynodioecious-gynomonoecious sexual system in section Psammophilae is closer to hermaphroditism or gynomonoecy than gynodioecy.

Dataset Information

Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain.

Publications

Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain.

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