Profound inhibition of CD73-dependent formation of anti-inflammatory adenosine in B cells of SLE patients.

ABSTRACT:

Background

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that leads to a breakdown of tolerance to self-antigens resulting in inflammation and organ damage. The anti-inflammatory activity of CD73-derived adenosine is well documented, however, its role in SLE pathogenesis is unknown.

Methods

Human peripheral blood immune cells were obtained from adult SLE patients (SLE) and healthy controls (HC). Expression and activity of purinergic ectoenzymes were assessed by qRT-PCR, flow cytometry and HPLC. Genes encoding purinergic ectoenzymes in SLE patients were analysed with targeted DNA sequencing.

Findings

Among circulating immune cells (both in HC and SLE), CD73 was most highly expressed on B cells, which was mirrored by high enzymatic activity only in

SUBMITTER: Hesse J 

PROVIDER: S-EPMC8524755 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature