Quantitative LC-MS/MS uncovers the regulatory role of autophagy in immune thrombocytopenia.

ABSTRACT:

Background

Immune thrombocytopenia (ITP) is an autoimmune haemorrhagic disease whose pathogenesis is associated with bone marrow megakaryocyte maturation disorder and destruction of the haematopoietic stem cell microenvironment.

Methods

In this study, we report the qualitative and quantitative profiles of the ITP proteome. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was conducted to elucidate the protein profiles of clinical bone marrow mononuclear cell (BMMC) samples from ITP patients and healthy donors (controls). Gene Ontology (GO) and Kyoto Encyclopaedia Genes and Genome (KEGG) pathway analyses were performed to annotate the differentially expressed proteins. A protein-protein interaction (PPI) network was constructed with the BLAST online database. Target

SUBMITTER: Sun RJ 

PROVIDER: S-EPMC8524881 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature