DNA methylation-calling tools for Oxford Nanopore sequencing: a survey and human epigenome-wide evaluation.

ABSTRACT:

Background

Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads. Here, we assess the performance of different methylation-calling tools to provide a systematic evaluation to guide researchers performing human epigenome-wide studies.

Results

We compare seven analytic tools for detecting DNA methylation from nanopore long-read sequencing data generated from human natural DNA at a whole-genome scale. We evaluate the per-read and per-site performance of CpG methylation prediction across different genomic contexts, CpG site coverage, and computational resources consumed by each tool. The seven

SUBMITTER: Liu Y 

PROVIDER: S-EPMC8524990 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature