Identification of colon tumor marker NKD1 via integrated bioinformatics analysis and experimental validation.

ABSTRACT:

Background

Colorectal cancer is an important death-related disease in the worldwide. However, specific colon cancer tumor markers currently used for diagnosis and treatment are few. The purpose of this study is to screen the potential colon cancer markers by bioinformatics and verify the results with experiments.

Methods

Gene expression data were downloaded from two different databases: TCGA database and GEO datasets, which were then analyzed by two different methods (difference analysis and WGCNA method). Venn and PPI analysis obtained the potential core genes, which were then performed the GO enrichment and KEGG pathway analysis. Expressions levels of NKD1 in colon carcinoma tissues were further confirmed by immunohistochemical staining and western blot assays. Moreover, t

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8525156 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature