Oral Immunization With a Plant HSP90-SAG1 Fusion Protein Produced in Tobacco Elicits Strong Immune Responses and Reduces Cyst Number and Clinical Signs of Toxoplasmosis in Mice.

ABSTRACT: Plant 90kDa heat shock protein (HSP90) is a potent adjuvant that increases both humoral and cellular immune responses to diverse proteins and peptides. In this study, we explored whether Arabidopsis thaliana HSP90 (AtHsp81.2) can improve the immune effects of a Toxoplasma gondii surface antigen 1 (SAG1). We designed two constructs containing the sequence of mature antigen (SAG1_m), from aa₇₇ to aa_322, and B- and T-cell antigenic epitope-containing SAG1_HC, from aa₂₂₁ to aa₃₁₉ fused to AtHsp81.2 sequence. When comparing the transient expression in Nicotiana tabacum X-27-8 leaves, which overexpress the suppressor helper component protease HC-Pro-tobacco etch virus (TEV), to that in N. benthamiana leav

SUBMITTER: Sanchez-Lopez EF 

PROVIDER: S-EPMC8525317 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature