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Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application.

ABSTRACT: In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA designs, or Cas proteins, but can also be a platform for DNA-free gene editing. To date, the latter approach has been applied to numerous crops, particularly those with complex genomes, a long juvenile period, a tendency for heterosis, and/or self-incompatibility. Protoplast regeneration is thus a key step in DNA-free gene editing. In this report, we review the history and some future prospects for protoplast technology, including protoplast transfection, transformation, fusion, regeneration, and current protoplast applications in CRISPR/Cas-based breeding.

SUBMITTER: Yue JJ

PROVIDER: S-EPMC8525356 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application.

Yue Jin-Jun JJ Yuan Jin-Ling JL Wu Fu-Hui FH Yuan Yu-Hsuan YH Cheng Qiao-Wei QW Hsu Chen-Tran CT Lin Choun-Sea CS

Frontiers in genome editing 20210811

In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA designs, or Cas proteins, but can also be a platform for DNA-free gene editing. To date, the latter approach has been applied to numerous crops, particularly those with complex genomes, a long juvenile period, a tendency for heterosis, and/or self-i ...[more]

PMID: 34713263

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