A Universal System of CRISPR/Cas9-Mediated Gene Targeting Using All-in-One Vector in Plants.

ABSTRACT: Homologous recombination-mediated genome editing, also called gene targeting (GT), is an essential technique that allows precise modification of a target sequence, including introduction of point mutations, knock-in of a reporter gene, and/or swapping of a functional domain. However, due to its low frequency, it has been difficult to establish GT approaches that can be applied widely to a large number of plant species. We have developed a simple and universal clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated DNA double-strand break (DSB)-induced GT system using an all-in-one vector comprising a CRISPR/Cas9 expression construct, selectable marker, and GT donor template. This system enabled introduction of targeted point mutations

SUBMITTER: Nishizawa-Yokoi A 

PROVIDER: S-EPMC8525384 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature