No association between cataract surgery and mitochondrial DNA damage with age-related macular degeneration in human donor eyes.

ABSTRACT:

Purpose

To determine whether age-related macular degeneration (AMD) severity or the frequency of retinal pigment epithelium mitochondrial DNA lesions differ in human donor eyes that have undergone cataract surgery compared to phakic eyes.

Methods

Eyes from human donors aged ≥ 55 years were obtained from the Minnesota Lions Eye Bank. Cataract surgery status was obtained from history provided to Eye Bank personnel by family members at the time of tissue procurement. Donor eyes were graded for AMD severity using the Minnesota Grading System. Quantitative PCR was performed on DNA isolated from macular punches of retinal pigment epithelium to quantitate the frequency of mitochondrial DNA lesions in the donor tissue. Univariable and multivariable analyses were performed to evaluat

SUBMITTER: Armbrust KR 

PROVIDER: S-EPMC8525771 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature