Neutrophil-Derived Proteases Contribute to the Pathogenesis of Early Diabetic Retinopathy.

ABSTRACT:

Purpose

Previous studies indicate that leukocytes, notably neutrophils, play a causal role in the capillary degeneration observed in diabetic retinopathy (DR), however, the mechanism by which they cause such degeneration is unknown. Neutrophil elastase (NE) is a protease released by neutrophils which participates in a variety of inflammatory diseases. In the present work, we investigated the potential involvement of NE in the development of early DR.

Methods

Experimental diabetes was induced in NE-deficient mice (Elane-/-), in mice treated daily with the NE inhibitor, sivelestat, and in mice overexpressing human alpha-1 antitrypsin (hAAT+). Mice were assessed for diabetes-induced retinal superoxide generation, inflammation, leukostasis, and capillary degeneration.

Results

SUBMITTER: Lessieur EM 

PROVIDER: S-EPMC8525836 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature