Mapping of a Subgingival Dual-Species Biofilm Model Using Confocal Raman Microscopy.

ABSTRACT: Techniques for continuously monitoring the formation of subgingival biofilm, in relation to the determination of species and their accumulation over time in gingivitis and periodontitis, are limited. In recent years, advancements in the field of optical spectroscopic techniques have provided an alternative for analyzing three-dimensional microbiological structures, replacing the traditional destructive or biofilm staining techniques. In this work, we have demonstrated that the use of confocal Raman spectroscopy coupled with multivariate analysis provides an approach to spatially differentiate bacteria in an in vitro model simulating a subgingival dual-species biofilm. The present study establishes a workflow to evaluate and differentiate bacterial species in a dual-species in vit

SUBMITTER: Kriem LS 

PROVIDER: S-EPMC8525910 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature