Overexpression of the maize WRKY114 gene in transgenic rice reduce plant height by regulating the biosynthesis of GA.

ABSTRACT: WRKYs represent an important family of transcription factors that are widely involved in plant development, defense regulation and stress response. Transgenic rice that constitutively expressed ZmWRKY114 had shorter plant height and showed less sensitivity to gibberellic acid (GA₃). Further investigation proved that transgenic rice accumulated lower levels of bioactive GAs than that in wild-type plants. Application of exogenous GA₃ fully rescued the semi-dwarf phenotype of ZmWRKY114 transgenic plants. Transcriptome and qRT-PCR analyses indicated that the expression of OsGA2ox4, encoding the repressor of GA biosynthesis, was markedly increased. Electrophoretic mobility shift assay and dual-luciferase reporter assay indicated that ZmWRKY114 directly

SUBMITTER: Fang X 

PROVIDER: S-EPMC8525977 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature