A high-throughput screening identifies ZNF418 as a novel regulator of the ubiquitin-proteasome system and autophagy-lysosomal pathway.

ABSTRACT: The ubiquitin-proteasome system (UPS) and autophagy-lysosomal pathway (ALP) are two major protein degradation pathways in eukaryotic cells. Initially considered as two independent pathways, there is emerging evidence that they can work in concert. As alterations of UPS and ALP function can contribute to neurodegenerative disorders, cancer and cardiac disease, there is great interest in finding targets that modulate these catabolic processes. We undertook an unbiased, total genome high-throughput screen to identify novel effectors that regulate both the UPS and ALP. We generated a stable HEK293 cell line expressing a UPS reporter (Ub^G76V-mCherry) and an ALP reporter (GFP-LC3) and screened for genes for which knockdown increased both Ub^G76V-mCherry intensity and GFP-LC3

SUBMITTER: Singh SR 

PROVIDER: S-EPMC8526018 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature