[An optimized protocol of meniscus cell extraction for single-cell RNA sequencing].

ABSTRACT:

Objective

To optimize the protocol of meniscus cell extraction to enhance the efficiency of cell suspension preparation and maintain a high cell viability for single-cell RNA sequencing.

Methods

We compared the efficiency of the routine cell extraction methods (short-time digestion and long-time digestion) and the optimized protocol for obtaining meniscus cell suspensions by evaluating the cell number obtained and the cell viability. Single-cell RNA sequencing datasets were analyzed to evaluate the stability of the cell suspension prepared using the optimized protocol. The reliability of the optimized protocol was assessed by comparing the single-cell RNA sequencing dataset obtained by the optimized protocol with published single-cell RNA sequencing datasets of the meniscus.

SUBMITTER: Chen N 

PROVIDER: S-EPMC8526306 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature