[Expression of RUNX2/LAPTM5 in MC3T3-E1 osteoblastic cells with induced mineralization].

ABSTRACT:

Objective

To investigate the association of the expressions of RUNX2/LAPTM5 with osteogenesis and lysosomes in osteoblastic cells during mineralization induction.

Methods

MC3T3- E1 cells cultured in osteogenic induction medium was examined for mineralization and osteogenic differentiation using Alizarin red staining and alkaline phosphatase (ALP) staining, respectively. RT-qPCR and Western blotting were used to detect the mRNA and protein expressions of Runx2 and LAPTM5 in the cells during osteogenic induction for 5 days. The effects of overexpression and interference of RUNX2/ LAPTM5 on the expressions of ALP and osteocalcin (OCN) in the cells were examined with Western blotting.

Results

MC3T3- E1 cells cultured in osteogenic induction medium showed an increased numb

SUBMITTER: Xing L 

PROVIDER: S-EPMC8526321 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature