Dark Respiration Measurement from Arabidopsis Shoots.

ABSTRACT: Dark respiration refers to experimental measures of leaf respiration in the absence of light, done to distinguish it from the photorespiration that occurs during photosynthesis. Dark aerobic respiration reactions occur solely in the mitochondria and convert glucose molecules from cytoplasmatic glycolysis and oxygen into carbon dioxide and water, with the generation of ATP molecules. Previous methods typically use oxygen sensors to measure oxygen depletion or complicated and expensive photosynthesis instruments to measure CO₂ accumulation. Here, we provide a detailed, step-by-step approach to measure dark respiration in plants by recording CO₂ fluxes of Arabidopsis shoot and root tissues. Briefly, plants are dark acclimated for 1 hour, leaves and roots are excis

SUBMITTER: Fonseca JP 

PROVIDER: S-EPMC8526368 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature