NRF2-mediated signaling is a master regulator of transcription factors in bovine granulosa cells under oxidative stress condition.

ABSTRACT: Transcription factors (TFs) are known to be involved in regulating the expression of several classes of genes during folliculogenesis. However, the regulatory role of TFs during oxidative stress (OS) is not fully understood. The current study was aimed to investigate the regulation of the TFs in bovine granulosa cells (bGCs) during exposure to OS induced by H₂O₂ in vitro. For this, bGCs derived from ovarian follicles were cultured in vitro till their confluency and then treated with H₂O₂ for 40 min. Twenty-four hours later, cells were subjected to various phenotypic and gene expression analyses for genes related to TFs, endoplasmic reticulum stress, apoptosis, cell proliferation, and differentiation markers. The bGCs exhibited higher reactive oxy

SUBMITTER: Taqi MO 

PROVIDER: S-EPMC8526460 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature