Decreased Calcium-Sensing Receptor Expression Controls Calcium Signaling and Cell-To-Cell Adhesion Defects in Aged Skin.

ABSTRACT: The calcium-sensing receptor (CaSR) drives essential calcium ion (Ca²⁺) and E-cadherin‒mediated processes in the epidermis, including differentiation, cell-to-cell adhesion, and epidermal barrier homeostasis in cells and in young adult mice. We now report that decreased CaSR expression leads to impaired Ca²⁺ signal propagation in aged mouse (aged >22 months) epidermis and human (aged >79 years, donor age) keratinocytes. Baseline cytosolic Ca²⁺ concentrations were higher, and capacitive Ca²⁺ entry was lower in aged than in young keratinocytes. As in Casr-knockout mice (^EpidCaSR^-/-), decreased CaSR expression led to decreased E-cadherin and phospholipase C-γ expression and to a compensatory upregulation of STIM1. Pretreatment

SUBMITTER: Celli A 

PROVIDER: S-EPMC8526647 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature