MCAs in Arabidopsis are Ca²⁺-permeable mechanosensitive channels inherently sensitive to membrane tension.

ABSTRACT: Mechanosensitive (MS) ion channels respond to mechanical stress and convert it into intracellular electric and ionic signals. Five MS channel families have been identified in plants, including the Mid1-Complementing Activity (MCA) channel; however, its activation mechanisms have not been elucidated in detail. We herein demonstrate that the MCA2 channel is a Ca²⁺-permeable MS channel that is directly activated by membrane tension. The N-terminal 173 residues of MCA1 and MCA2 were synthesized in vitro, purified, and reconstituted into artificial liposomal membranes. Liposomes reconstituted with MCA1(1-173) or MCA2(1-173) mediate Ca²⁺ influx and the application of pressure to the membrane reconstituted with MCA2(1-173) elicits channel currents. This channel is also activ

SUBMITTER: Yoshimura K 

PROVIDER: S-EPMC8526687 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature