Glucocorticoid-Responsive Transcription Factor Kruppel-Like Factor 9 Regulates fkbp5 and Metabolism.

ABSTRACT: Krüppel-like factor 9 (Klf9) is a feedforward regulator of glucocorticoid receptor (GR) signaling. Here we show that in zebrafish klf9 is expressed with GR-dependent oscillatory dynamics in synchrony with fkbp5, a GR target that encodes a negative feedback regulator of GR signaling. We found that fkbp5 transcript levels are elevated in klf9 ^-/- mutants and that Klf9 associates with chromatin at the fkbp5 promoter, which becomes hyperacetylated in klf9 ^{-/ -} mutants, suggesting that the GR regulates fkbp5 via an incoherent feedforward loop with klf9. As both the GR and Fkbp5 are known to regulate metabolism, we asked how loss of Klf9 affects metabolic rate and gene expression. We found that klf

SUBMITTER: Gans IM 

PROVIDER: S-EPMC8526736 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature