Sulfation of O-glycans on Mucin-type Proteins From Serous Ovarian Epithelial Tumors.

ABSTRACT: Despite sulfated O-linked glycans being abundant on ovarian cancer (OC) glycoproteins, their regulation during cancer development and involvement in cancer pathogenesis remain unexplored. We characterized O-glycans carrying sulfation on galactose residues and compared their expression with defined sulfotransferases regulated during OC development. Desialylated sulfated oligosaccharides were released from acidic glycoproteins in the cyst fluid from one patient with a benign serous cyst and one patient with serous OC. Oligosaccharides characterized by LC-MSⁿ were identified as core 1 and core 2 O-glycans up to the size of decamers and with 1 to 4 sulfates linked to GlcNAc residues and to C-3 and/or C-6 of Gal. To study the specificity of the potential ovarian sulfotransferases inv

SUBMITTER: Thomsson KA 

PROVIDER: S-EPMC8527052 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature