[Exploring the therapeutic mechanism of quercetin for heart failure based on network pharmacology and molecular docking].

ABSTRACT:

Objective

To investigate the molecular mechanism of quercetin in the treatment of heart failure (HF) based on network pharmacology and molecular docking.

Methods

Quercetin and HF-related targets were obtained using TCMSP, PharmMapper, CTD and GeneCards databases, and quercetin-HF intersection targets were obtained through the online website Venn; the protein interaction network was constructed and imported into Cytoscape 3.7.2 to identify the core targets of quercetin in the treatment of HF.GO and KEGG pathway enrichment analyses were performed using R package, and molecular docking was performed using Auto Dock Vina.The protein levels of AKT1, phospho-AKT(Ser473), eNOS, MMP9, and caspase-3 in quercetin-treated HF cell models were detected using protein immunoblotting.

Re

SUBMITTER: Tan X 

PROVIDER: S-EPMC8527225 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature