Differentially accessible, single copy sequences form contiguous domains along metaphase chromosomes that are conserved among multiple tissues.

ABSTRACT:

Background

During mitosis, chromatin engages in a dynamic cycle of condensation and decondensation. Condensation into distinct units to ensure high fidelity segregation is followed by rapid and reproducible decondensation to produce functional daughter cells. Factors contributing to the reproducibility of chromatin structure between cell generations are not well understood. We investigated local metaphase chromosome condensation along mitotic chromosomes within genomic intervals showing differential accessibility (DA) between homologs. DA was originally identified using short sequence-defined single copy (sc) DNA probes of < 5 kb in length by fluorescence in situ hybridization (scFISH) in peripheral lymphocytes. These structural differences between metaphase homologs are non-random

SUBMITTER: Hill SL 

PROVIDER: S-EPMC8527651 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature