Differential expression analysis using a model-based gene clustering algorithm for RNA-seq data.

ABSTRACT:

Background

RNA-seq is a tool for measuring gene expression and is commonly used to identify differentially expressed genes (DEGs). Gene clustering is used to classify DEGs with similar expression patterns for the subsequent analyses of data from experiments such as time-courses or multi-group comparisons. However, gene clustering has rarely been used for analyzing simple two-group data or differential expression (DE). In this study, we report that a model-based clustering algorithm implemented in an R package, MBCluster.Seq, can also be used for DE analysis.

Results

The input data originally used by MBCluster.Seq is DEGs, and the proposed method (called MBCdeg) uses all genes for the analysis. The method uses posterior probabilities of genes assigned to a cluster displaying

SUBMITTER: Osabe T 

PROVIDER: S-EPMC8527798 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature