Cellular fibronectin promotes deep vein thrombosis in diet-induced obese mice.

ABSTRACT:

Background

Overweight and obesity are significant risk factors for deep vein thrombosis (DVT). Cellular fibronectin containing extra domain A (Fn-EDA), an endogenous ligand for toll-like-receptor 4 (TLR4), contributes to thrombo-inflammation. The role of Fn-EDA in the modulation of DVT is not elucidated yet.

Objective

To determine whether Fn-EDA promotes DVT in the context of diet-induced obesity.

Methods

Wild-type (WT) and Fn-EDA-deficient mice were either fed control or high-fat (HF) diet for 12 weeks. DVT was induced by inferior vena cava (IVC) stenosis and evaluated after 48 hours. Cellular Fn-EDA levels in the plasma of venous thromboembolism (VTE) patients were measured by sandwich ELISA.

Results

We found that cellular Fn-EDA levels were significantly ele

SUBMITTER: Dhanesha N 

PROVIDER: S-EPMC8527852 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature