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Real-time imaging of cell-surface proteins with antibody-based fluorogenic probes.

ABSTRACT: Cell-surface proteins, working as key agents in various diseases, are the targets for around 66% of approved human drugs. A general strategy to selectively detect these proteins in a real-time manner is expected to facilitate the development of new drugs and medical diagnoses. Although brilliant successes were attained using small-molecule probes, they could cover a narrow range of targets due to the lack of suitable ligands and some of them suffer from selectivity issues. We report herein an antibody-based fluorogenic probe prepared via a two-step chemical modification under physiological conditions, to fulfill the selective recognition and wash-free imaging of membrane proteins, establishing a modular strategy with broad implications for biochemical research and for therapeutics.

SUBMITTER: Wang W

PROVIDER: S-EPMC8528012 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Real-time imaging of cell-surface proteins with antibody-based fluorogenic probes.

Wang Wenchao W Zhang Ying Y Zhao Hong H Zhuang Xinlei X Wang Haoting H He Kaifeng K Xu Wanting W Kang Yu Y Chen Shuqing S Zeng Su S Qian Linghui L

Chemical science 20210916 40

Cell-surface proteins, working as key agents in various diseases, are the targets for around 66% of approved human drugs. A general strategy to selectively detect these proteins in a real-time manner is expected to facilitate the development of new drugs and medical diagnoses. Although brilliant successes were attained using small-molecule probes, they could cover a narrow range of targets due to the lack of suitable ligands and some of them suffer from selectivity issues. We report herein an an ...[more]

PMID: 34777767

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