Glomerular endothelial cells and podocytes can express CD80 in patients with minimal change disease during relapse.

ABSTRACT:

Background

Urinary CD80 has emerged as potential biomarker in idiopathic nephrotic syndrome (INS). However, its cellular source remains controversial. The aim of the study was to assess whether CD80 is truly expressed by glomerular cells in INS patients during relapse and in the LPS mouse model of podocyte injury.

Methods

The presence of CD80 in glomeruli was evaluated by combining immunostaining, immunogold labeling, and in situ hybridization techniques.

Results

CD80 was present along the surface of glomerular endothelial cells (GEC) and rarely in podocytes in six of nine minimal change disease (MCD) patients in relapse, two of eleven patients with focal segmental glomerulosclerosis in relapse, and absent in controls. In mice, CD80 was upregulated at mRNA and protein

SUBMITTER: Cara-Fuentes G 

PROVIDER: S-EPMC8528162 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature