Cancer Cell-Derived Exosomes Promote HCC Tumorigenesis Through Hedgehog Pathway.

ABSTRACT:

Purpose

Hepatocellular carcinoma (HCC) accounts for more than 80% of primary liver cancers and is one of the leading causes of cancer-related death in many countries. Cancer cell-derived exosomes are shown to mediate communications between cancer cells and the microenvironment, promoting tumorigenesis. Hedgehog signaling pathway plays important roles in cancer development of HCC.

Methods

Exosomes were isolated from culture medium of HCC cell lines PLC/PRF/5 and MHCC-97H and were found to promote cancer cell growth measured with cell proliferation and colony formation assay. HCC cells cultured with cancer cell-derived exosome had increased cancer stem cell (CSC) population demonstrated by increased cell sphere formation CSC marker expressions. Hedgehog protein Shh was found t

SUBMITTER: Li L 

PROVIDER: S-EPMC8529041 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature