Single-particle mass analysis of intact ribosomes by mass photometry and Orbitrap-based charge detection mass spectrometry.

ABSTRACT: Standard methods for mass analysis measure ensembles of thousand to millions of molecules. This approach enables analysis of monodisperse recombinant proteins, whereas some heterogeneous protein assemblies pose a significant challenge, whereby co-occurring stoichiometries, sub-complexes, and modifications hamper analysis using native mass spectrometry. To tackle the challenges posed by mass heterogeneity, single-particle methods may come to the rescue. Recently, two such approaches have been introduced, namely, mass photometry (MP) and Orbitrap-based charge detection mass spectrometry (CDMS). Both methods assess masses of individual molecules, albeit adhering to distinct physical principles. To evaluate these methods side by side, we analyzed a set of ribosomal particles, representing poly

SUBMITTER: Lai SH 

PROVIDER: S-EPMC8529500 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature