Customizable Aptamer Transducer Network Designed for Feed-Forward Coupling.

ABSTRACT: Solution-based biosensors that utilize aptamers have been engineered in a variety of formats to detect a range of analytes for both medical and environmental applications. However, since aptamers have fixed base sequences, incorporation of aptamers into DNA strand displacement networks for feed-forward signal amplification and processing requires significant redesign of downstream DNA reaction networks. We designed a novel aptamer transduction network that releases customizable output domains, which can then be used to initiate downstream strand displacement reaction networks without any sequence redesign of the downstream reaction networks. In our aptamer transducer (AT), aptamer input domains are independent of output domains within the same DNA complex and are reacted with a fuel strand

SUBMITTER: Hachigian T 

PROVIDER: S-EPMC8529589 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature