Xylonolactonase from Caulobacter crescentus Is a Mononuclear Nonheme Iron Hydrolase.

ABSTRACT: Caulobacter crescentus xylonolactonase (Cc XylC, EC 3.1.1.68) catalyzes an intramolecular ester bond hydrolysis over a nonenzymatic acid/base catalysis. Cc XylC is a member of the SMP30 protein family, whose members have previously been reported to be active in the presence of bivalent metal ions, such as Ca²⁺, Zn²⁺, and Mg²⁺. By native mass spectrometry, we studied the binding of several bivalent metal ions to Cc XylC and observed that it binds only one of them, namely, the Fe²⁺ cation, specifically and with a high affinity (K_d = 0.5 μM), pointing out that Cc XylC is a mononuclear iron protein. We propose that bivalent metal cations also promote the reaction nonenzymatically by stabilizing a s

SUBMITTER: Paakkonen J 

PROVIDER: S-EPMC8529709 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature