Red blood cells and their releasates compromise bone marrow-derived human mesenchymal stem/stromal cell survival in vitro.

ABSTRACT:

Purpose

The use of bone marrow aspirate (BMA) and bone marrow aspirate concentrate (BMC) in the treatment of inflammatory orthopedic conditions has become a common practice. The therapeutic effect of BMA/BMC is thought to revolve primarily around the mesenchymal stem/stromal cell (MSC) population residing within the nucleated cell fraction. MSCs have the unique ability to respond to site of injury via the secretion of immunomodulating factors, resolving inflammation in diseased joints. Recently, the importance of hematocrit (HCT) in BMC has been debated, as the potential impact on MSC function is unknown. In the present study, we investigate MSC health over a short time-course following exposure to a range of HCT and red blood cell releasate (RBC_rel) conditions.

Metho

SUBMITTER: Dregalla RC 

PROVIDER: S-EPMC8529765 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature