Functionally Mature CD1c⁺ Dendritic Cells Preferentially Accumulate in the Inflammatory Arthritis Synovium.

ABSTRACT:

Objective

To examine the role of synovial CD1c⁺DCs in patients with Inflammatory Arthritis (IA) with a specific focus on the transcriptional and maturation signatures that govern their function.

Methods

RNA sequencing was performed on healthy control (HC) peripheral blood (PB), IA PB, and IA synovial fluid (SF) CD1c⁺DCs. Multiparametric flow-cytometry and SPICE analysis were used to examine site [SF and Synovial Tissue (ST) CD1c+DCs] and disease specific characteristics of CD1c⁺DCs, while functional assays such as antigen processing, activation, and MMP production were also performed.

Results

Increased frequency of CD1c⁺DCs (p<0.01) with a concomitant increase in CD80, CCR7 (p<0.01), and CXCR3 (p<0.05) expression was identif

SUBMITTER: Canavan M 

PROVIDER: S-EPMC8529992 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature