Live-imaging rate-of-kill compound profiling for Chagas disease drug discovery with a new automated high-content assay.

ABSTRACT: Chagas disease, caused by the protozoan intracellular parasite Trypanosoma cruzi, is a highly neglected tropical disease, causing significant morbidity and mortality in central and south America. Current treatments are inadequate, and recent clinical trials of drugs inhibiting CYP51 have failed, exposing a lack of understanding of how to translate laboratory findings to the clinic. Following these failures many new model systems have been developed, both in vitro and in vivo, that provide improved understanding of the causes for clinical trial failures. Amongst these are in vitro rate-of-kill (RoK) assays that reveal how fast compounds kill intracellular parasites. Such assays have shown clear distinctions between the compounds that failed in clinical trials and the standard of care. Howev

SUBMITTER: Svensen N 

PROVIDER: S-EPMC8530327 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature