Bifunctional TGF-β trap/IL-15 protein complex elicits potent NK cell and CD8⁺ T cell immunity against solid tumors.

ABSTRACT: Advances in immunostimulatory and anti-immunosuppressive therapeutics have revolutionized cancer treatment. However, novel immunotherapeutics with these dual functions are not frequently reported. Here we describe the creation of a heterodimeric bifunctional fusion molecule, HCW9218, constructed using our soluble tissue factor (TF)-based scaffold technology. This complex comprises extracellular domains of the human transforming growth factor-β (TGF-β) receptor II and a human interleukin-15 (IL-15)/IL-15 receptor α complex. HCW9218 can be readily expressed in CHO cells and purified using antibody-based affinity chromatography in a large-scale manufacturing setting. HCW9218 potently activates mouse natural killer (NK) cells and CD8⁺ T cells in vitro and in vivo to enhance cell pro

SUBMITTER: Liu B 

PROVIDER: S-EPMC8531151 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature